Visualization method for the direct or indirect detection of the reaction between a specific binding agent and the corresponding acceptor substance in blot overlay assays

Méthode de visualisation pour la détection directe ou indirecte de la réaction entre un agent de liaison spécifique et la substance acceptrice correspondante dans des essais "blot overlay"

Verfahren zur optischen Anzeige der direkten oder indirekten Bestimmung der Reaktion zwischen einem spezifischen Bindungsmittel und der korrespondierenden Akzeptorsubstanz in "Blot overlay"-Testverfahren

Abstract

A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristic for the colloidal metal particles used or quantitatively determined following art-known spectopho- tometric procedures such as densitometry.

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Patent Citations (6)

    Publication numberPublication dateAssigneeTitle
    EP-0007654-A1February 06, 1980Akzo N.V.Process for the detection and/or determination of components of the reaction between a specific binding protein and the corresponding bindable substance in an aqueous test sample; freeze-dried reagent and test kit to be used in the process
    EP-0173629-A1March 05, 1986Cytogen CorporationAntibody-metal ion complexes
    FR-2353854-A1December 30, 1977Technion Res & Dev FoundationMethode et reactif d'analyse par liaison specifique
    US-4203724-AMay 20, 1980Mitsubishi Chemical Industries LimitedMethod and apparatus for the measurement of antigens and antibodies
    US-4208185-AJune 17, 1980Mitsubishi Chemical Industries LimitedMethod and apparatus for the measurement of antigens and antibodies
    US-4420558-ADecember 13, 1983Janssen Pharmaceutica N.V.Bright field light microscopic method of enumerating and characterizing subtypes of white blood cells and their precursors

NO-Patent Citations (6)

    Title
    ANALYTICAL BIOCHEMISTRY, vol. 124, 1982; p. 396
    CHEMICAL ABSTRACTS, vol. 101, 10th September 1984, page 376, abstract no. 87638q, Columbus, Ohio, US; U. ZUR NIEDEN et al.: "Dictyosomes participate in the intracellular pathway of storage proteins in developing Vicia faba cotyledons", & EUR. J. CELL BIOL. 1984, 34(1), 9-17
    CHEMICAL ABSTRACTS, vol. 101, 5th November 1984, page 496, abstract no. 168633m, Columbus, Ohio, US; Y.H. HSU: "Immunogold for detection of antigen on nitrocellulose paper", & ANAL. BIOCHEM. 1984, 142(1), 221-5
    CHEMICAL ABSTRACTS, vol. 102, 14th July 1985, page 437, abstract no. 94024k, Columbus, Ohio, US; M. MOEREMANS et al.: "Sensitive visualization of antigen-antibody reactions in dot and blot immune overlay assays with immunogold and immunogold/silver staining", & J. IMMUNOL. METHODS 1984, 74(2), 353-60
    CHEMICAL ABSTRACTS, vol. 102, 7th January 1985, page 311, abstract no. 163185c, Columbus, Ohio, US; M. MOEREMANS et al.: "Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes", & ANAL. BIOCHEM. 1985, 145(2), 315-21
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES USA, vol. 76, no. 9, 1979; p. 4350

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