Proteine decouplante et procedes d'utilisation

Novel uncoupling protein and methods of use

Abstract

La présente invention concerne, d'une part une protéine découplante, dénommée UCP-3 par le déposant, et qui s'exprime dans les muscles squelettiques et les tissus adipeux bruns, et d'autre part des molécules d'acide nucléique codant pour la protéine de l'invention. L'invention concerne également, d'une part des procédés de recherche de composés régulant l'expression et l'activité de la protéine UCP-3, et d'autre part des traitements d'affections ou d'états où doit intervenir une régulation de la thermogenèse ou de la synthèse de l'adénosine-triphosphate respiratoire. Les états concernés sont notamment l'obésité, le diabète, l'hyperthermie maligne per-anesthésique, et la fièvre. L'invention concerne enfin la constitution de lignées cellulaires exprimant la protéine UCP-3.
A novel uncoupling protein, which we have designated UCP-3, that is expressed in skeletal muscle and brown adipose tissue (BAT), and nucleic acid molecules which encode for said novel protein, are described. Methods of screening for compounds that regulate the expression and the activity of UCP-3 are described, as well as methods of treating diseases or conditions in which the regulation of thermogenesis or respiratory ATP synthesis is desired. Such conditions include obesity, diabetes, malignant hyperthermia, and fever. The construction of cell lines that express UCP-3 is also described.

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NO-Patent Citations (3)

    Title
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    URHAMMER S. A., ET AL.: "ORGANISATION OF THE CODING EXONS AND MUTATIONAL SCREENING OF THE UNCOUPLING PROTEIN 3 GENE IN SUBJECTS WITH JUVENILE-ONSET OBESITY.", DIABETOLOGIA, SPRINGER, BERLIN, DE, vol. 41., 1 January 1998 (1998-01-01), BERLIN, DE, pages 241 - 244., XP002914181, ISSN: 0012-186X, DOI: 10.1007/s001250050897
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